FlyBase Allele Report: Dmel\ush[2]

General Information

Symbol

Dmel\ush²

Species

D. melanogaster

Name

FlyBase ID

FBal0017649

Feature type

allele

Associated gene

Dmel\ush

Associated Insertion(s)

Carried in Construct

Also Known As

ush^IIA102, ush^IIA

Key Links

Allele class

loss of function allele

hypomorphic allele - genetic evidence

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Biryukova and Heitzler, 2008, Cubadda et al., 1997, Frank and Rushlow, 1996)

hypomorphic allele - genetic evidence

(Goldman-Levi et al., 1996)

loss of function allele

(Epps and Tanda, 1998)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2L)BSC107)

(Miller et al., 2020, Kahsai et al., 2016)

lethal - all die during embryonic stage

(Biryukova and Heitzler, 2008)

lethal - all die during embryonic stage | recessive

(Frank and Rushlow, 1996, Goldman-Levi et al., 1996)

some die during embryonic stage

(Biryukova and Heitzler, 2008)

some die during embryonic stage | recessive

(Frank and Rushlow, 1996, Goldman-Levi et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

ush² homozygous embryos are U-shaped.

ush²/+ flies have the wildtype number of dorsocentral bristles.

(Biryukova and Heitzler, 2008)

ush² mutant embryos exhibit an increased number of cardioblasts.

(Sellin et al., 2006)

Homozygous mutants show an increase in cardial cells during embryonic development. There is also an increase in the number of eve expressing pericardial cells seen - from three per cluster in wild-type, to 5-6 per cluster in homozygous mutants.

(Fossett et al., 2000)

In ush² mutant embryos the dorsal trunk is continuous and secondary branching is indistinguishable from wild-type.

(Wilk et al., 2000)

Apoptotic cells are visible in the amnioserosa of stage 11-12 homozygous embryos.

(Byars et al., 1999)

The posterior half of the abdomen is folded back on the anterior half, due to a failure of germband retraction, resulting in an eponymous phenotype. At full germband extension A8 is often opposite A1 or T3. Head involution fails and major head parts are missing. The head is pulled posteriorly away from the anterior of the vitelline membrane, leaving empty the anterior ~40% of the egg. There is often a hole in the cuticle spanning the juxtaposed head and tail. Some components of the head skeleton are absent or rudimentary, including the dorsal arms, dorsal bridge and vertical plate. The filzkorper are often widely separated. Anti-β-tubulin staining reveals a premature loss of amnioserosa cells after stage 11. The amnioserosa undergoes programmed cell death.

(Frank and Rushlow, 1996)

Homozygous embryos exhibit strong germ band retraction defects.

(Goldman-Levi et al., 1996)

Germband retraction and dorsal closure fail to occur in homozygous embryos.

(Strecker et al., 1995)

Nondefective in gonad assembly.

(Warrior, 1994)

Does not interact with RpII140^wimp maternal effect.

(Parkhurst and Ish-Horowicz, 1991)

Suppression of tor^13D embryos: increase in proportion of embryos that formed cuticle and decrease in proportion of embryos that formed empty sacs.

(Strecker et al., 1991)

strong

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

ush² has phenotype, enhanceable by srp^neo45

(Goldman-Levi et al., 1996)

ush² has phenotype, enhanceable by tup¹

(Goldman-Levi et al., 1996)

Enhancer of

Statement

Reference

ush² is an enhancer of phenotype of srp^neo45

(Goldman-Levi et al., 1996)

ush² is an enhancer of phenotype of tup¹

(Goldman-Levi et al., 1996)

ush² is an enhancer of phenotype of peb^hnt-1

(Goldman-Levi et al., 1996)

Suppressor of

Statement

Reference

ush[+]/ush² is a suppressor | partially of posterior scutellar bristle | adult stage phenotype of CtBP^UAS.cSa, Scer\GAL4^ap-md544

(Stern et al., 2009)

ush[+]/ush² is a suppressor | partially of anterior scutellar bristle | adult stage phenotype of CtBP^UAS.cSa, Scer\GAL4^ap-md544

(Stern et al., 2009)

ush[+]/ush² is a suppressor | partially of posterior dorsocentral bristle | adult stage phenotype of CtBP^UAS.cSa, Scer\GAL4^ap-md544

(Stern et al., 2009)

ush[+]/ush² is a suppressor | partially of anterior dorsocentral bristle | adult stage phenotype of CtBP^UAS.cSa, Scer\GAL4^ap-md544

(Stern et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

ush²/+ dominantly suppresses the missing thoracic bristle phenotype of CtBP^Scer\UAS.cSa, Scer\GAL4^ap-md544 flies.

(Stern et al., 2009)

ush²/+; CtBP^rev19/+ flies have the wildtype number of dorsocentral bristles.

(Biryukova and Heitzler, 2008)

Germ band retraction defects are more severe than the single homozygote when in combination with tup¹, peb^hnt-1 or srp^neo45.

(Goldman-Levi et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

2508

ush² cn¹ bw¹ speck¹/CyO

Kyoto

107372

ush² cn¹ bw¹ speck¹/CyO

Notes on Origin

Discoverer

Comments

Null allele. Complements lwr⁰²⁸⁵⁸.

(Epps and Tanda, 1998)

Both midgut primordia extend normally along the visceral mesoderm and the yolk is completely engulfed by the endoderm.

(Reuter et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

ush²

(Stern et al., 2009, Sellin et al., 2006)

ush^IIA102

(Biryukova and Heitzler, 2008, Epps and Tanda, 1998, Cubadda et al., 1997, Harbecke and Lengyel, 1995, Parkhurst and Ish-Horowicz, 1991, Strecker et al., 1991)

ush^IIA108

(Goldman-Levi et al., 1996)

ush^IIA

(Frank and Rushlow, 1996, Strecker et al., 1995, Warrior, 1994, Reuter et al., 1993, Tearle and Nusslein-Volhard, 1987)

ush^IIa

Name Synonyms

Secondary FlyBase IDs

References (24)

Report Sections

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