FlyBase Allele Report: Dmel\srp[neo45]

General Information

Symbol

Dmel\srp^neo45

Species

D. melanogaster

Name

FlyBase ID

FBal0011058

Feature type

allele

Associated gene

Dmel\srp

Associated Insertion(s)

P{hsneo}srp^neo45

Carried in Construct

Also Known As

srp^AS, srp^p282

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Heitzler et al., 1996, Rehorn et al., 1996, Sam et al., 1996, Cooley et al., 1988)

Progenitor genotype

Associated Insertion(s)

P{hsneo}srp^neo45

Cytology

Description

P{hsneo} insertion within 5' flanking sequences.

(Rehorn et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:15,983,653..15,986,705 [-]

(Cho et al., 2002, Spradling et al., 1999, Goldman-Levi et al., 1996, Goldman-Levi et al., 1996, Heitzler et al., 1996, Rehorn et al., 1996, Sam et al., 1996, Cooley et al., 1988)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

amnioserosa

(Goldman-Levi et al., 1996)

embryonic/larval crystal cell

(Milchanowski et al., 2004)

embryonic/larval hemocyte

(Cho et al., 2002, Kramerova et al., 2000)

embryonic/larval hemocyte (with srp³)

(Stramer et al., 2005)

embryonic/larval hemocyte | embryonic stage

(Rehorn et al., 1996, Sam et al., 1996)

embryonic/larval hemocyte | embryonic stage (with srp²)

(Rehorn et al., 1996)

embryonic/larval hemocyte | embryonic stage (with srp³)

(Rehorn et al., 1996)

embryonic/larval lymph gland

(Han and Olson, 2005)

larval glial cell

(Sears et al., 2003)

larval tracheocyte | precursor

(Cho et al., 2002)

macrochaeta | dorsal

(Goldman-Levi et al., 1996)

mitotic domain 1 | extended germ band stage

(Goldman-Levi et al., 1996)

presumptive embryonic/larval central nervous system

(Sears et al., 2003)

ventral midline of embryo

(Cho et al., 2002)

Detailed Description

Statement

Reference

In srp^neo45 embryos, the lymph gland does not form but most cardioblasts and pericardial cells do form.

(Han and Olson, 2005)

srp³/srp^neo45 embryos lack all hemocytes yet show an entirely normal time course of wound reepithelialization.

(Stramer et al., 2005)

The average number of crystal cells per embryo is reduced in homozygous stage 13-14 embryos compared to wild type.

(Milchanowski et al., 2004)

Mutant embryos lack macrophages and have defects in the central nervous system axon scaffold (which has a rounded appearance). Increased numbers of repo-positive glial cells are present at the midline compared to wild type, while the glia associated with the longitudinal tracts appear more dispersed than in wild type.

(Sears et al., 2003)

Mutants embryos lack blood cells. During stages 11-13 have some missing tracheal cells. Also a small group of cells near each tracheal visceral branch, and a small group ventral midline cells in each segment, possibly midline glia.

(Cho et al., 2002)

Mutants lack hemocytes.

(Kramerova et al., 2000)

Homozygous embryos exhibit weak germ band retraction defects, one or no ventral setae remain on the dorsal aspect of the embryo. Kr staining reveals abnormalities in the amnioserosa.

(Goldman-Levi et al., 1996)

Semi-viable.

(Heitzler et al., 1996)

Homozygous embryos exhibit severe reduction in number of mature haemocytes and srp²/srp^neo45 and srp³/srp^neo45 transheterozygous embryos are devoid of any mature haemocytes.

(Rehorn et al., 1996)

The fat body appears normal in mutant embryos. Hemocytes are missing.

(Sam et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

srp^neo45 has phenotype, enhanceable by ush²

(Goldman-Levi et al., 1996)

Enhancer of

Statement

Reference

srp^neo45 is an enhancer of phenotype of ush²

(Goldman-Levi et al., 1996)

srp^neo45 is an enhancer of phenotype of peb^hnt-1

(Goldman-Levi et al., 1996)

Suppressor of

Statement

Reference

srp^neo45/srp[+] is a suppressor of lamellocyte | larval stage phenotype of ush^VX22

(Sorrentino et al., 2007)

srp^neo45/srp[+] is a suppressor of hemocyte | larval stage phenotype of ush^VX22

(Sorrentino et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Lamellocyte production is drastically reduced in ush^VX22/+, srp^neo45/+ double heterozygous mutant larvae, but not quite to wild-type levels. The number of non-lamellocyte cells is reduced to almost half the wild-type value.

(Sorrentino et al., 2007)

Germ band retraction defects are more severe than the single homozygote when in combination with ush² or peb^hnt-1.

(Goldman-Levi et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

srp⁰¹⁵⁴⁹

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59020

w^*; P{hsneo}srp^neo45 e¹/TM6B, Tb¹

Notes on Origin

Discoverer

A. Spradling.

Cooley et al.

(Cooley et al., 1988)

Comments

Complements: tara⁰³⁸⁸¹. Complements: Akt1⁰⁴²²⁶. Complements: l(3)05203⁰⁵²⁰³. Complements: l(3)08724⁰⁸⁷²⁴.

(BDGP Project Members, 1994-1999)

P{hsneo} insertion specifically affects the srp function required for development of the haemocytes, probably a regulatory region which is essential for the anterior mesodermal srp expression.

(Rehorn et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

Ins(3R)neo45

P282

(Goldman-Levi et al., 1996)

Srp^neo45

(Brown et al., 2020)

l(3)neo45

(Spradling et al., 1999)

l(3)neo45¹

srp⁴⁵

(Heitzler et al., 1996)

srp^AS282

(Goldman-Levi et al., 1996)

srp^AS

(Moussalem et al., 2021, Matsubayashi et al., 2017, Stramer et al., 2008, Stramer et al., 2005, Cho et al., 2002, Rehorn et al., 1996)

srp^P282

(Sam et al., 1996)

srp^neo45

(Töpfer et al., 2019, Gajewski et al., 2007, Sorrentino et al., 2007, Muratoglu et al., 2006)

srp^p282

(Kramerova et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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