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Christensen, S., Cook, K., Cook, K. (2008.4.15). Isolation and characterization of Df(3L)BSC413. 
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FBrf0204510
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC413
Date: 	Tue, 15 Apr 2008  14:18:31  -0400  ( 19:18  BST)
Isolation and characterization of Df(3L)BSC413
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC413 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d01629 and PBac{WH}MICAL-like[f05713]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; P{XP}d01629/PBac{WH}MICAL-like[f05713] males. The males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC413 from the segment 
of P{XP}d01629 to the left of its FRT site and the segment of 
PBac{WH}MICAL-like[f05713] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al. The cytological breakpoints of Df(3L)BSC413 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 69C2;69F5. Df(3L)BSC413 failed to complement Ptp69D[1] and 
Df(3L)ED4483.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    English
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)