Open Close
Christensen, S., Cook, K., Cook, K. (2008.4.15). Isolation and characterization of Df(3L)BSC413. 
FlyBase ID
Publication Type
Personal communication to FlyBase
PubMed ID
PubMed Central ID
Text of Personal Communication
From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC413
Date: 	Tue, 15 Apr 2008  14:18:31  -0400  ( 19:18  BST)
Isolation and characterization of Df(3L)BSC413
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC413 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d01629 and PBac{WH}MICAL-like[f05713]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; P{XP}d01629/PBac{WH}MICAL-like[f05713] males. The males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC413 from the segment 
of P{XP}d01629 to the left of its FRT site and the segment of 
PBac{WH}MICAL-like[f05713] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al. The cytological breakpoints of Df(3L)BSC413 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 69C2;69F5. Df(3L)BSC413 failed to complement Ptp69D[1] and 
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
Associated Information
Associated Files
Other Information
Secondary IDs
    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Data From Reference
    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)