Isolation and characterization of Df(3R)BSC478
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC478 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f07611 and P{XP}d11464. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f07611/P{XP}d11464 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC478 from the segment of PBac{WH}f07611 to the left of its FRT site and the segment of P{XP}d11464 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC478 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 85A5;85B8. Df(3R)BSC478 failed to complement hb12, pyd1 and Df(3R)Exel6150.