Isolation and characterization of Df(3R)BSC422
Bloomington Stock Center
Indiana University
Df(3R)BSC422 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f03036 and P{XP}Antpd06610. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f03036/P{XP}Antpd06610 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC422 from the segment of PBac{WH}f03036 to the left of its FRT site and the segment of P{XP}Antpd06610 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC422 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 84A5; 84B2. Df(3R)BSC422 failed to complement zen7, Antp1 and Dfd14.