Isolation and characterization of Df(3R)BSC516
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC516 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f06764 and P{XP}CG17838d09234. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f06764/P{XP}CG17838d09234 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC516 from the segment of PBac{WH}f06764 to the left of its FRT site and the segment of P{XP}CG17838d09234 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC516 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 92C6;92F13. Df(3R)BSC516 failed to complement Stat92E06346 and bon21B, and Df(3R)BSC516 heterozygotes show a Hairless (H) phenotype.