Isolation and characterization of Df(3R)BSC518
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC518 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f00705 and P{XP}d04966. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f00705/P{XP}d04966 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC518 from the segment of PBac{WH}f00705 to the left of its FRT site and the segment of P{XP}d04966 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC518 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 92E8;92F13. Df(3R)BSC518 failed to complement Stat92E06346 and bon21B, and Df(3R)BSC518 heterozygotes show a Hairless (H) phenotype.