Isolation and characterization of Df(3L)BSC558
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC558 was isolated as a FLP recombinase-induced recombination event involving P{XP}d08056 and PBac{WH}f05189. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d08056/PBac{WH}f05189 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC558 from the segment of P{XP}d08056 to the left of its FRT site and the segment of PBac{WH}f05189 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d08056 to be Release 3 genomic coordinate 15459825 on chromosome arm 3L. The Gene Disruption project determined the insertion site of P{XP}d08056 to be Release 3 genomic coordinate 15459907 on arm 3L. This corresponds to 71D3 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}f05189 on the Release 5 map is 71E1. Consequently, the cytological breakpoints of Df(3L)BSC558 are predicted to be 71D3;71E1. Df(3L)BSC558 failed to complement Df(3L)BSC442 and Df(3L)ED217.