Isolation and characterization of Df(3R)BSC566
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC566 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG7431f05682 and P{XP}Ssdpd03345. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG7431f05682/P{XP}Ssdpd03345 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC566 from the segment of PBac{WH}CG7431f05682 to the left of its FRT site and the segment of P{XP}Ssdpd03345 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC566 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 90C2;90F6. Df(3R)BSC566 failed to complement Df(3R)ED5785, sr1 and htld07110.