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Christensen, S., Cook, K., Cook, K. (2008.7.11). Isolation and characterization of Df(1)BSC572. 
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FBrf0205020
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From: 	Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(1)BSC572
Date: 	Fri, 11 Jul 2008  13:23:33  -0400  ( 18:23  BST)
Isolation and characterization of Df(1)BSC572
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC572 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d09152 and PBac{RB}e04308. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{XP}d09152/PBac{RB}e04308; MKRS, P{hsFLP}86E/+ females crossed to 
Binsinscy/Y males. These females were heat shocked as larvae as 
described in Parks et al., Nature Genetics 36: 288-292, 2004 
(FBrf0175003). This cross and crosses in preceding and succeeding 
generations maintained the original genetic background of the 
Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC572 from the segment of P{XP}d09152 
to the left of its FRT site and the segment of PBac{RB}e04308 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. with the substitution 
of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or 
RB3' minus primer in the Hybrid PCR protocol in the Supplementary 
Methods. Exelixis, Inc. determined the insertion site of 
PBac{RB}e04308 to be Release 3 genomic coordinate 10834026 on the X 
chromosome. The Gene Disruption project determined the insertion site 
of PBac{RB}e04308 to be Release 3 genomic coordinate 10834192 on the 
X chromosome. This corresponds to 10A4 on the Release 3 and Release 5 
genome maps. The predicted position of P{XP}d09152 on the Release 5 
map is 9F8. Consequently, the cytological breakpoints of Df(1)BSC572 
are predicted to be 9F8;10A4. It failed to complement v[1].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    English
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (2)