Open Close
Christensen, S., Cook, K., Cook, K. (2008.7.11). Isolation and characterization of Df(1)BSC572. 
FlyBase ID
Publication Type
Personal communication to FlyBase
PubMed ID
PubMed Central ID
Text of Personal Communication
From: 	Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(1)BSC572
Date: 	Fri, 11 Jul 2008  13:23:33  -0400  ( 18:23  BST)
Isolation and characterization of Df(1)BSC572
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC572 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d09152 and PBac{RB}e04308. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{XP}d09152/PBac{RB}e04308; MKRS, P{hsFLP}86E/+ females crossed to 
Binsinscy/Y males. These females were heat shocked as larvae as 
described in Parks et al., Nature Genetics 36: 288-292, 2004 
(FBrf0175003). This cross and crosses in preceding and succeeding 
generations maintained the original genetic background of the 
Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC572 from the segment of P{XP}d09152 
to the left of its FRT site and the segment of PBac{RB}e04308 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. with the substitution 
of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or 
RB3' minus primer in the Hybrid PCR protocol in the Supplementary 
Methods. Exelixis, Inc. determined the insertion site of 
PBac{RB}e04308 to be Release 3 genomic coordinate 10834026 on the X 
chromosome. The Gene Disruption project determined the insertion site 
of PBac{RB}e04308 to be Release 3 genomic coordinate 10834192 on the 
X chromosome. This corresponds to 10A4 on the Release 3 and Release 5 
genome maps. The predicted position of P{XP}d09152 on the Release 5 
map is 9F8. Consequently, the cytological breakpoints of Df(1)BSC572 
are predicted to be 9F8;10A4. It failed to complement v[1].
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
Associated Information
Associated Files
Other Information
Secondary IDs
    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Data From Reference
    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (2)