PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
Exelixis, Inc. determined the insertion site of PBac{RB}e04308 to be Release 3 genomic coordinate 10834026 on the X chromosome. The Gene Disruption project determined the insertion site of PBac{RB}e04308 to be Release 3 genomic coordinate 10834192 on the X chromosome. This corresponds to 10A4 on the Release 3 and Release 5 genome maps.
5.971
e04308