Isolation and characterization of Df(2R)BSC603
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC603 was isolated as a FLP recombinase-induced recombination event involving P{XP}bsd01376 and PBac{WH}CG13579f00432. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}bsd01376/PBac{WH}CG13579f00432 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC603 from the segment of P{XP}bsd01376 to the left of its FRT site and the segment of PBac{WH}CG13579f00432 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC603 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 60C7;60D1. Df(2R)BSC603 failed to complement Df(2R)ED4065 and Mov34k08003.