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Christensen, S., Cook, K., Cook, K. (2008.10.20). Isolation and characterization of Df(3L)BSC649. 
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From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, kaufmanXXXX, Stacey Christensen <sjchristXXXX>, Kim Cook <ruacookXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC649
Date: 	Mon, 20 Oct 2008  09:19:55  -0400  ( 14:19  BST)
Isolation and characterization of Df(3L)BSC649
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC649 was isolated as a FLP recombinase-induced recombination 
event involving PBac{RB}e03222 and P{XP}roqd08799. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to 
P{hsFLP}1, y1 w1118; PBac{RB}e03222/P{XP}roqd08799 males. The 
males were heat shocked as larvae as described in Parks et al., 
Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and 
crosses in preceding and succeeding generations maintained the 
original genetic background of the Exelixis insertion stocks 
(Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). 
The recombination event generated the genetic element 
P+PBac{XP5.RB3}BSC649 from the segment of PBac{RB}e03222 to the left 
of its FRT site and the segment of P{XP}roqd08799 to the right of 
its FRT site. Its presence was verified using the PCR methods and 
primers described in Parks et al. with the substitution of the primer 
5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus 
primer in the Hybrid PCR protocol in the Supplementary Methods. The 
cytological breakpoints of Df(3L)BSC649 predicted from the Release 5 
genomic coordinates of the transposable element insertions sites are 
72E1;72E4. Df(3L)BSC649 failed to complement Df(3L)ED220 and Df(3L)BSC555.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    Aberrations (3)
    Insertions (3)
    Transgenic Constructs (1)