Isolation and characterization of Df(2R)BSC702
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC702 was isolated as a FLP recombinase-induced recombination event involving P{XP}d08982 and PBac{WH}Ipk1f07534. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d08982/PBac{WH}Ipk1f07534 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC702 from the segment of P{XP}d08982 to the left of its FRT site and the segment of PBac{WH}Ipk1f07534 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC702 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 57A2;57B3. Df(2R)BSC702 failed to complement exu1, insck12405, Df(2R)BSC400 and Df(2R)BSC404.