Isolation and characterization of Df(3R)BSC728
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC728 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG4221d08178 and PBac{RB}Mhcle03696. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}CG4221d08178/PBac{RB}Mhcle03696 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.RB5}BSC728 from the segment of P{XP}CG4221d08178 to the left of its FRT site and the segment of PBac{RB}Mhcle03696 to the right of its FRT site. The cytological breakpoints of Df(3R)BSC728 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 89A8;89B2. Df(3R)BSC728 failed to complement Df(3R)Exel7327 and srp3.