Annotations that are candidates for removal: 1) CG1162 shows no evidence of coding in that I do not see any wobble upon EvoPrinting. 2) EvoPrinting CG31179 yields no trace of a wobble, and I doubt whether it is a gene. There are a couple ESTs but these do not coincide well with the transcript. Evidence of ORFs in currently annotated UTRs: 1) In EvoPrinting Trl, I found indication of coding sequence in the 3'UTR. There is an ORF in this sequence. 2) The following sequence is in the 3'UTR of dan, but exhibits a wobble signature, as indicated by the EvoPrint given below. There are no stops in the sequence. Since dan is in the intron of another gene it could belong to that other gene. GAaAAGCCaAAGTTGGCgCCcacGGAGCCAGCCACGGATAcagagatagaggatagcccagacggtgctctgcacg atgcagagccaggacccggtgtcctggctacagatgcaaatgcagataccgccgagggctcgacgaggtgccatgt ctatgcgccggccgtctaccgatcgtcgGCcACcCTaCTCAGCTCcCTGTTCTTCCCtCCCTACGAATTTGTACAt TGTGACCTCGAcGAtGACCCcGAcGAcTGCCAgATcACGGGCGAGTAcCAGCAGTaCGAcGAAcTgAgcTCcAGCA G 3) In the undefined 3'UTR of Octbeta2R a wobble signature recognized by EvoPrinter aacattacgagtaaatcagccgacgcatttgccgGCATATTAATTTTacAAATTAATGGAacac ttTaATTGCATTaaATtGAttccacccatccggctggcatcgattgattcgattcgcatccttacaccagtcgca ccatttgcaccatttgcaccatttACACaTGaCTcgaggccgtcGAGGCgGCggcaTTGTTgTTcTTgTTCCATT TGTTGATgTACTCCATGGGTATtTCaATcTGgATCTCCTGCACATCgTCgcccgccaagcaatcgacattggatt tgcccttgcccgcatccttgcccgtatcctttgcatccttcgactccctgccatccttggcggcagctgccaatt gtccgcccaggcgggatccgccCGCCGCCAGCCGCTGCTGCTGCTGgGCgatggGgGTCAGcAGCGCCTCaTCCC TGTA Other corrections: 1) I have found a potential ORF in an intron <up>of sv</up>. Every non-conserved base in the EvoPrint, given below, fits in with wobble. It could be a short functional peptide. You might look and see if there is other evidence for it. ATGGAcCTaTCTACAGCaTAtCGCTACAAcCAgAAcATGATGGAATAcTAcACATGTAA 2) Dif is annotated incorrectly at UCSC, and a blast of a solid ORF yields no ORF in FlyBase BLAST. I attach the EvoPrint. The ORF is in the reverse directions. gaaaatatctcgttatcccattttgtcttcaaatcagttatccgtaacccaattcatctactccaAgCCa tTtGcCTCcGCcAgccgcCgtGCtATtTCAtccGcactcaaaaaatcggtcgtatccactccaccagttgctgct ccaccttcgccgcactccccgccaaatggtggtTCcGGaCttTTGCTGGTgTAcAaaGCAAAGTGtTCcGCcTCG GAgAGaTCCACaCTGGGATCcagcacaacACCaTAGGCtCCCTCGCAcAgATTCTTGATGCTCTGcctgtcgGCg ATGGCaAtCAatTCCATaTTGCCgTCaCTtATGGGaATATCcGCCAgtTGcAGaGTGCTCAGGGTCTTcCtaTCc AGcGGTCGaCCgTAaCTaAGGGTGCTGGCATTcGAGTAGCTCTCcGGtCgtAGCAaaCTGTTCTCGCTaTCGCCt CCCTTCAatGGTATGTGaATaACATCcGCaTCCAGgTgGGCaGGtCttTTaCCCGAtACaCTGCTcGAaCTaCGA CcgactggcttgccatttcgaccctcactttttcccagtggCTGCAtcgatcttgtgGAGCtgCGaTTGGGATCt cCCAGgGaaAAGTGcgcaacgcttggttccctactgccggtgggtgttgtggctttcgatccccctggtagttgc ttattggggctgccaccaggtgtcagagattctTCgtCaTccgtCTTGCTccTtCGGCGaGAaAAGAAtctCGAG AAGAAtccctgaCGCTTCTTtGGCGAaGCACTgGGcgtcggttgccttTTgGTGGGaCTtTGaTCaGGgGTtCGC ATTATaATtataGGCGAagGtgtaGTtCtatTGGAgCTtAgGCTGCCGTTtCgCGAgCGgctcacagaaattgaa tcactgacagcaCTCAGaTCCTGgGAGTTtctctgcGGtttcggtggtaagggaggtaatctctgatctgaaact ggggaaGGaGTtCgaGGtGGcaatGGcAGgGGCGGtGAGgctGGgGGcGTGGCtGGtGTCTGaGCagcgTTcACT TTgATCCTGGgaGCtGGtGGATTtGTcAGcACCACCAgtTCgtCaTCATCtAGCTGaCcaAAcGGATTGGTgggt ggataaatgggggctgcttgtttgctttctttgggcaatcctatgtctAtgGGaTTaTTaaaggctatttGtAGg CTcGTaTAtGTgGCtGTCTCaTCGAAgtTtTCaTCtAaATCCAtaGAgGCCTGGGCaCTGGCtgaaaccatttcc ggttctttttcattttccggtagctctttcacgGCcGGATTCTCgAGgGTaTccctgcgagaaccattggccaaa agaccgtgggcttccTCGTCcAacTGGTTCGTTATGGTCGTaTTGGCtGTCAaGCTGCTgACaTTgGTaAatgct GTtTGGGCcGTGCTaTTCGAtAGgGattGcGTGTCCCCgTTCTCCACcGTCAGGCTaTCcGTTCtCTCgAACTCG CTGCTCTTCATCCACTCggTgATtGTttCCACaTTGgacAGgGGtCGcGatTGcAGCTGCGGaTGCTGCAGaATC TCCTCCATcGGCGTgGAcGTCTGgGTGCAGTCaTCGGCcAGtTGgGGCAaCAatTGCGGaACcTCCAGCTCtATT TCCATaTCtGGTTCgTTaTCgTTaTCCGTGTACTCgGTgacagtgccaccatccatttcgtgcgattcacccctc tgacgcattTGCAttTTCTTgACgGCtATcTCGAAtTCgTTgAGaGTtcCCGAaCTCTGttggtcaTCGGAtgaa ACCGTGTTaTTaTccTCcTCcAGaTCcAtCGGtGGGCAcGAgTAtTTCGTGGCcGTcGTtTCGCTgTCtAgGGAA AGgATCTGCTGGAAcACgTCCAgtTCCTGgcggCGtTTCAaCTTtCGCTGtAGCCGggCAAAgGTTActgTACCT GGATTcGGgTAGTACTCGAAcgGCAGtgggGcaCTcGTCGCtccaTCcGAGGGTCtcactagtttcaGcTcCAcC TGgaaggggggaaaaagtggagttagttctatttcattcgtgcaaaaaatttaaactcggaaaattaatgttttc ggaagtacaagtattcgattaatggtacttagagcgcaaatgctattttcagtttattttacgttattaaatttg ttttt 3) A miniexon in Mbs from splice sites AG.....GT as below AGCTgAGtGATTCcACgGAaAGCTCACACttgACcAGtCTtTCCGAaAGCGAAGGT 4)A small ORF with wobble signature above pollux: ATGTTcActgtgccgtttcacCGGGAcACCATcCTGAC caCcTCcGTCAGCGAtGCATCgGTgCTcAAcAAGAGtCGgGCCGTAtGTAgagattgggatccagtatccagtat Attccgtatattcctattccgtattgcccgcatcgaaccactaacccctaaattgagcttgtgtaa There is a perfect wobble signature in the EvoPrint.