Isolation and characterization of Df(2R)BSC777
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC777 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f04393 and P{XP}d07949. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}f04393/P{XP}d07949 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC777 from the segment of PBac{WH}f04393 to the left of its FRT site and the segment of P{XP}d07949 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The Gene Disruption project determined the insertion site of P{XP}d07949 to be Release 3 genomic coordinate 18487680 on arm 2R. This corresponds to Release 5 coordinate  2R:19331635 . The insertion site of PBac{WH}f04393 is Release 5 genomic coordinate 18997609 on arm 2R. Consequently, the molecular breakpoints of Df(2R)BSC777 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:18997609 ;19331635 and the cytological breakpoints predicted from these coordinates are 59C4;59E1. Df(2R)BSC777 failed to complement Df(2R)BSC769 and Df(2R)BSC698.