Isolation and characterization of Df(3L)BSC797
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC797 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG5199f05676 and P{XP}d05199. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG5199f05676/P{XP}d05199 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC797 from the segment of PBac{WH}CG5199f05676 to the left of its FRT site and the segment of P{XP}d05199 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3L)BSC797 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3L:20445923 ;20942833 and the cytological breakpoints predicted from these coordinates are 77C3;78A1. Df(3L)BSC797 failed to complement Df(3L)BSC452 and kniri-1.