Isolation and characterization of Df(3L)BSC839
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC839 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f03016 and P{XP}CG5130d01634. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f03016/P{XP}CG5130d01634 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC839 from the segment of PBac{WH}f03016 to the left of its FRT site and the segment of P{XP}CG5130d01634 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3L)BSC839 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3L:20313247 ;20486308 and the cytological breakpoints predicted from these coordinates are 77B4;77C6. Df(3L)BSC839 failed to complement DNAprimL5 and fbl1.