Griffin, R., Sustar, A., Bonvin, M., Binari, R., del Valle Rodriguez, A., Hohl, A.M., Bateman, J.R., Villalta, C., Heffern, E., Grunwald, D., Bakal, C., Desplan, C., Schubiger, G., Wu, C.T., Perrimon, N. (2009). The twin spot generator for differential Drosophila lineage analysis.  Nat. Methods 6(8): 600--602.

FBrf0208347

Research paper

In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.

PMC2720837 (PMC) (EuropePMC)