We have identified CG15287 as a likely candidate for ms(2)34Fe.
The evidence is this:
1) The molecularly defined deficiencies Df(2L)ED8185 and Df(2L)ED8186 are genetically wb-, ms(2)34Fe-, l(2)34Fc+. ED8185 and ED8186 delete 450kb from cenG1a to wb, CG15287 and just 30 bps into CG33090 i.e. the right hand breakpoint of these deletions (the insertion site of P{RS5}5-HA-2418) is 30bps within CG33090. 5-HA-2418 itself is male fertile.
(Additionally Df(2L)b78j is genetically wb-, ms(2)34Fe-, l(2)34Fc+.)
2) The molecularly defined deletion Df(2L)FDD-0042597 which deletes CG33090 (and CG15286, CG18125 and mTTF) is homozygous viable and fertile.
3) Df(2L)A217 is genetically wb+, ms(2)34Fe- (and extends 300kb proximally to Adh). This places ms(2)34Fe proximal of wb.
John Roote
University of Cambridge