Microarray analysis of Df(3R)sbd104 Kim Cook, Eric Spana and Kevin Cook DNA samples from Df(3R)sbd104 (FBab0010058) heterozygotes were compared to samples from wild type flies by Comparative Genomic Hybridization microarrays at the Duke Model System Genomics Unit as described in Erickson and Spana, 2006 (/reports/FBrf0193934.html). Corning CGAP slides spotted with the AROS Drosophila V1.1.1 ~70 nucleotide oligo set from Eurofins MWG Operon (www.operon.com) were used for the analysis. Most annotated genes were represented by a single oligo (denoted by a DM number). Sequences present at one copy in deletion heterozygotes are detected by lower relative fluorescence when compared to sequences present in two copies in wild type flies. The left Df(3R)sbd104 breakpoint lies within Sb in the range 3R:11970815..11971397 (R5) (predicted cytology: 89B6) based on the following evidence. The gene order at the left Df(3R)sbd104 end is Akt1 (FBgn0010379), Sb (FBgn0003319), CG5903 (FBgn0038400). A sequence within CG5903 (DM00008713, 3R:11971397..11971465 (R5)) was deleted. A sequence within Sb (DM00007446, 3R:11970747..11970815 (R5)) was not deleted, but published complementation data indicate Sb is disrupted (see /reports/FBab0010058.html). A sequence within Akt1 (DM00013636, 3R:11925293..11925361 (R5)) was not deleted. The right Df(3R)sbd104 breakpoint lies within Gyc-89Da or Gyc-89Db or in the region between them, and lies in the range 3R:12300639..12306048 (R5) (predicted cytology: 89B17) based on the following evidence. The gene order at the left Df(3R)sbd104 end is Gyc-89Da (FBgn0038435), Gyc-89Db (FBgn0038436), Dhfr (FBgn0004087). A sequence within Gyc-89Da (DM00003237, 3R:12300571..12300639 (R5)) was deleted. A sequence within Gyc-89Db (DM00003238, 3R:12306048..12306116 (R5)) was not deleted, but Gyc-89Db may have been disrupted. A sequence within Dhfr (DM00003239, 3R:12307240..12307308 (R5)) was not deleted. The rest of the microarray data are consistent with genes between Sb and Gyc-89Da being deleted in Df(3R)sbd104.
