FB2026_03 , released September 17, 2026
Reference Report
Open Close
Reference
Citation
Cook, K., Cook, K. (2010.5.7). Isolation and characterization of Df(2R)BSC883. 
FlyBase ID
FBrf0210930
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
Df(2R)BSC883 was isolated as a FLP recombinase-induced recombination event involving P{XP}d02243 and PBac{WH}CG11048f00140. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d02243/PBac{WH}CG11048f00140 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC883 from the segment of P{XP}d02243 to the left of its FRT site and the segment of PBac{WH}CG11048f00140 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(2R)BSC883 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:15519525 ;16130288 and the cytological breakpoints predicted from these coordinates are 56E1;56F11. Df(2R)BSC883 failed to complement hrg10.
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)