FB2026_02 , released June 18, 2026
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Cook, K., Cook, K. (2010.5.7). Isolation and characterization of Df(3R)BSC887. 
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FBrf0210934
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Personal communication to FlyBase
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Df(3R)BSC887 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG6006d09073 and PBac{WH}CG14894f04937. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}CG6006d09073/PBac{WH}CG14894f04937 males. The males were heat shocked as larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC887 from the segment of P{XP}CG6006d09073 to the left of its FRT site and the segment of PBac{WH}CG14894f04937 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3R)BSC887 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:11983756 ;12268648 and the cytological breakpoints predicted from these coordinates are 89B6;89B16. Df(3R)BSC887 failed to complement Df(3R)Exel7329.
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    English
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    Aberrations (2)
    Insertions (3)
    Transgenic Constructs (1)