Isolation and characterization of Df(2L)BSC891
Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC891 was isolated as a FLP recombinase-induced recombination event involving P{XP}escld01514 and PBac{WH}CG31760f00461. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y1 w1118; P{XP}escld01514/PBac{WH}CG31760f00461 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC891 from the segment of P{XP}escld01514 to the left of its FRT site and the segment of PBac{WH}CG31760f00461 to the right of its FRT site. The breakpoints of Df(2L)BSC891 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 2L:11990983 ;12170401 and the cytological breakpoints predicted from these coordinates are 33B5;33D2. Df(2L)BSC891 failed to complement prd8 and Df(2L)ED775.
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Kevin Cook, Ph.D.
Bloomington Drosophila Stock Center
Department of Biology
Indiana University
1001 E. Third St.
Bloomington, IN 47405-7005
