Ting, C.Y., Gu, S., Guttikonda, S., Lin, T.Y., White, B.H., Lee, C.H. (2011). Focusing Transgene Expression in Drosophila by Coupling Gal4 With a Novel Split-LexA Expression System.  Genetics 188(1): 229--233.

FBrf0213643

Research paper

Here we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.

PMC3120155 (PMC) (EuropePMC)