Halder, P., Chen, Y.C., Brauckhoff, J., Hofbauer, A., Dabauvalle, M.C., Lewandrowski, U., Winkler, C., Sickmann, A., Buchner, E. (2011). Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain.  PLoS ONE 6(12): e29352.

FBrf0217036

Research paper

The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.

PMC3244249 (PMC) (EuropePMC)