Fisher, S.Q., Weck, M., Landers, J.E., Emrich, J., Middleton, S.A., Cox, J., Gentile, L., Parish, C.A. (2012). Evidence that the kinesin light chain domain contains tetratricopeptide repeat units. J. Struct. Biol. 177(3): 602--612.
FlyBase ID
FBrf0217804
Publication Type
Research paper
Abstract
Homology models were built for various length sequences of the kinesin-1 light chain (KLC) domain of Drosophila melanogaster and subjected to 200 ns of all-atom molecular dynamics. We also cloned, expressed and characterized these regions spectroscopically. Results confirm that KLC contains tetratricopeptide repeat units; a regular array of repeating 34-residue helix-loop-helix monomers. Experimental and computational evidence is provided confirming the stability and overall helicity of individual TPR repeats as well as individual TPR units incorporated into a multi-TPR structure.
