FB2026_03 , released September 17, 2026
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Berger, C. (2012.10.16). Neuroblast and neuron RNA-Seq tracks. 
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FBrf0219845
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Personal communication to FlyBase
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Text of Personal Communication 
Here are now the new WIG files  split into plus & minus strand.
Up to alignment it is as described in "Extended Experimental Procedures". Afterwards the BAM files are merged (see 2) with samtools. The strandedness is converted to "forward-forward". All aligned reads are piled up to bedGraphs with "genomeCoverageBed" from BEDTools and split into +/- strand. The bedGraphs are converted to 1-based variable wiggle files.
neuron.merge.plus.wig: 14336733 reads
neuron.merge.minus.wig: 14379053 reads
nb.merge.plus.wig: 67519599 reads
nb.merge.minus.wig: 70186187 reads
For the merged files following samples were used:
GSM949245    wild type neuroblast 1
GSM949246    wild type neuroblast 2 (tech. Repl. 1)
GSM949247    wild type neuroblast 2 (tech. Repl. 2)
GSM949248    wild type neuroblast 3 (tech. Repl. 1)
GSM949249    wild type neuroblast 3 (tech. Repl. 2)
GSM949250    wild type neuron 1 (tech. Repl. 1)
GSM949251    wild type neuron 1 (tech. Repl. 2)
GSM949252    wild type neuron 2
Dr. Christian Berger
Institute of Genetics
University of Mainz
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Related Publication(s)
Research paper

FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal.
Berger et al., 2012, Cell Rep. 2(2): 407--418 [FBrf0219320]

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File date: 2012.10.16 ; File size: 92982533 ; File format: wig ; File name: Berger.121016.nb.merge.minus.wig
File date: 2012.10.16 ; File size: 91746471 ; File format: wig ; File name: Berger.121016.nb.merge.plus.wig
File date: 2012.10.16 ; File size: 83225184 ; File format: wig ; File name: Berger.121016.neuron.merge.minus.wig
File date: 2012.10.16 ; File size: 83489263 ; File format: wig ; File name: Berger.121016.neuron.merge.plus.wig
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    English
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