The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Douglas Kim, Howard Hughes Medical Institute, Janelia Farm Research Campus.
P{20XUAS-IVS-GCaMP6s}, P{20XUAS-IVS-GCaMP6m} and P{20XUAS-IVS-GCaMP6f} encode high sensitivity green fluorescent calcium sensor protein variants under the control of UAS in the pJFRC7-20XUAS-IVS vector (see Pfeiffer et al., 2010, FBrf0212052 for vector information).
Variant names are based on decay kinetics -- GCaMP6s (slow), GCaMP6m (medium), and GCaMP6f (fast). In a Drosophila larval neuromuscular junction stimulation and imaging assay, they perform as follows relative to GCaMP5G (for GCaMP5G kinetics see Akerboom et al., 2012, FBrf0219564):
construct | 1 Hz deltaF/F0 | 5 Hz deltaF/F0 | 20 Hz deltaF/F0 | 80 Hz deltaF/F0 | 160 Hz deltaF/F0 | Decay time 20 Hz
GCaMP5G | 100% | 100% | 100% | 100% | 100% | 100%
GCaMP6s | 216% | 585% | 370% | 134% | 121% | 188%
GCaMP6m | 358% | 1079% | 419% | 112% | 104% | 144%
GCaMP6f | 160% | 213% | 175% | 111% | 113% | 62%
Sequences for the rat versions of these constructs can be found at Addgene (http://www.addgene.org/)
P{20XUAS-IVS-GCaMP6s}attP40 is a homozygous viable and fertile insertion on the second chromosome in the attP40 site at 25C6,  2L:5108448 .
P{20XUAS-IVS-GCaMP6f}attP40 is a homozygous viable and fertile insertion on the second chromosome in the attP40 site at 25C6,  2L:5108448 .
P{20XUAS-IVS-GCaMP6m}attP40 is a homozygous viable and fertile insertion on the second chromosome in the attP40 site at 25C6,  2L:5108448 .
PBac{20XUAS-IVS-GCaMP6s}VK00005 is a homozygous viable and fertile insertion on the third chromosome in an attP site at 75A10,  3L:17945208 .
PBac{20XUAS-IVS-GCaMP6m}VK00005 is a homozygous viable and fertile insertion on the third chromosome in an attP site at 75A10,  3L:17945208 .
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Annette Parks, Ph.D.
Bloomington Drosophila Stock Center
http://flystocks.bio.indiana.edu