Hiroi, M., Ohkura, M., Nakai, J., Masuda, N., Hashimoto, K., Inoue, K., Fiala, A., Tabata, T. (2013). Principal component analysis of odor coding at the level of third-order olfactory neurons in Drosophila. Genes Cells 18(12): 1070--1081.
FlyBase ID
FBrf0223819
Publication Type
Research paper
Abstract
Olfactory information in Drosophila is conveyed by projection neurons from olfactory sensory neurons to Kenyon cells (KCs) in the mushroom body (MB). A subset of KCs responds to a given odor molecule, and the combination of these KCs represents a part of the neuronal olfactory code. KCs are also thought to function as coincidence detectors for memory formation, associating odor information with a coincident punishment or reward stimulus. Associative conditioning has been shown to modify KC output. This plasticity occurs in the vertical lobes of MBs containing α/α' branches of KCs, which is shown by measuring the average Ca(2+) levels in the branch of each lobe. We devised a method to quantitatively describe the population activity patterns recorded from axons of >1000 KCs at the α/α' branches using two-photon Ca(2+) imaging. Principal component analysis of the population activity patterns clearly differentiated the responses to distinct odors.
