The following information accompanied a stock donated by Sharyn Endow, Duke University.
P{ncd-ncd.mRFP} (FBtp0053432) consists of the ncd promoter and ncd cDNA fused to mRFP1. The last four amino acid of wild-type ncd are -NFDK. This was changed to -NFLK-mRFP1. The construct was made in pP{CaSpeR-3}.
The mRFP1 sequence is at http://www.ncbi.nlm.nih.gov/nuccore/AF506027.1:
1 atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
61 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
121 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
181 ctgtcccctc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc
241 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
301 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
361 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
421 atgggctggg aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag
481 atcaagatga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc
541 tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac
601 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
661 cactccaccg gcgcctaa
P{ncd-ncd.mRFP}F1F1 is a homozygous viable and fertile, third chromosome insertion.