The following information accompanied stocks donated to the Bloomington Stock Center by Sven Huelsmann, University of Jyväskylä.
M{UASp-LifeAct.TagRFP.H} expresses the LifeAct actin-binding motif from Scer\ABP140 (FBgn0262977) linked via a polyserine linker to a C-terminal TagRFP tag under the control of UASp regulatory sequences. It has been codon optimized for Drosophila and it is an independent construct from the similar P{UASp-LifeAct-TagRFP} construct described by Zanet et al. (/reports/FBrf0218285.html).
M{UASp-LifeAct.TagRFP.H}ZH-51D is an insertion into M{3xP3-RFP.attP}ZH-51D at 51D9, 2R:10941803 (R5).
M{UASp-LifeAct.TagRFP.H}ZH-96E is an insertion into M{3xP3-RFP.attP'}ZH-96E at 96E10, 3R:21523617 (R5).
M{UASp-LifeAct.TagRFP-T} expresses the LifeAct actin-binding motif from Scer\ABP140 (FBgn0262977) linked via a polyserine linker to a C-terminal TagRFP-T tag (a photostable TagRFP variant) under the control of UASp regulatory sequences.
M{UASp-LifeAct.TagRFP-T}ZH-22A is an insertion into M{3xP3-RFP.attP'}ZH-22A at 22A2, 2L:1476459 (R5).
M{UASp-LifeAct.TagRFP-T}ZH-68E is an insertion into M{3xP3-RFP.attP'}ZH-68E at 68E1, 3L:11837236 (R5)