FB2026_03 , released September 17, 2026
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Huelsmann, S. (2014.12.18). M{UASp-LifeAct.TagRFP.H} and M{UASp-LifeAct.TagRFP-T} constructs and insertions. 
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FBrf0227286
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The following information accompanied stocks donated to the Bloomington Stock Center by Sven Huelsmann, University of Jyväskylä.
M{UASp-LifeAct.TagRFP.H} expresses the LifeAct actin-binding motif from Scer\ABP140 (FBgn0262977) linked via a polyserine linker to a C-terminal TagRFP tag under the control of UASp regulatory sequences. It has been codon optimized for Drosophila and it is an independent construct from the similar P{UASp-LifeAct-TagRFP} construct described by Zanet et al. (/reports/FBrf0218285.html).
M{UASp-LifeAct.TagRFP.H}ZH-51D is an insertion into M{3xP3-RFP.attP}ZH-51D at 51D9,  2R:10941803  (R5). 
M{UASp-LifeAct.TagRFP.H}ZH-96E is an insertion into M{3xP3-RFP.attP'}ZH-96E at 96E10,  3R:21523617  (R5).
M{UASp-LifeAct.TagRFP-T} expresses the LifeAct actin-binding motif from Scer\ABP140 (FBgn0262977) linked via a polyserine linker to a C-terminal TagRFP-T tag (a photostable TagRFP variant) under the control of UASp regulatory sequences.
M{UASp-LifeAct.TagRFP-T}ZH-22A is an insertion into M{3xP3-RFP.attP'}ZH-22A at 22A2,  2L:1476459  (R5).
M{UASp-LifeAct.TagRFP-T}ZH-68E is an insertion into M{3xP3-RFP.attP'}ZH-68E at 68E1,  3L:11837236  (R5)
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File date: 2014.12.18 ; File size: 38365 ; File format: pdf ; File name: UASp-LifeAct-TagRFP_and_UASp-LifeAct-TagRFP-T_constructs.pdf
File date: 2014.12.18 ; File size: 14779 ; File format: gb ; File name: pUASP-attB-lifeact-tagRFP.gb
File date: 2014.12.18 ; File size: 14760 ; File format: gb ; File name: pUASP-attB-lifeact-tagRFP_T.gb
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    English
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