The flanking sequences of the G505 GenExel line (FBti0100715) as determined by the GDP are: >G505-5 KS307057 <up>insertion position 29</up> GCGCGAATCAGCTGCTGGAACGAGTGTTGTTGGAAG >G505-3 KS307056 <up>insertion position 1</up> GTTGGAAGGCAGTGTTGTTGGAAGTGGCAGCGC (GenBank accessions are given in the FASTA header.) These map to a unique site in the D. melanogaster reference genome (assembly release 6): X:1375536 (+). This location at the 5' end of the AMPKalpha gene agrees with the map location in the published reference (FBrf0201353) that uses this insertion to generate mutations of AMPKalpha by imprecise excision.