Xu, H., Sepúlveda, L.A., Figard, L., Sokac, A.M., Golding, I. (2015). Combining protein and mRNA quantification to decipher transcriptional regulation. Nat. Methods 12(8): 739--742.
FlyBase ID
FBrf0229173
Publication Type
Research paper
Abstract
We combine immunofluorescence and single-molecule fluorescence in situ hybridization (smFISH), followed by automated image analysis, to quantify the concentration of nuclear transcription factors, number of transcription factors bound, and number of nascent mRNAs synthesized at individual gene loci. A theoretical model is used to decipher how transcription factor binding modulates the stochastic kinetics of mRNA production. We demonstrate this approach by examining the regulation of hunchback in the early Drosophila embryo.
