The lethal phenotype of P{lacW}Adk2k16120
Lily Kahsai Golden and Kevin Cook
Bloomington Drosophila Stock Center
P{lacW}k16120 (FBti0006368) is lethal in combination with Df(2R)BSC770 (FBab0045836), a deletion of 60A13;60B5 ( 2R:23949444 ;24046810, R6). This is consistent with disruption of Adk2 (FBgn0022708) by P{lacW} insertion causing lethality as suggested by sequence localization of the insertion to 60B4 ( 2R:24024374 , R6). 
P{lacW}k16120 was reported by the Berkeley Drosophila Genome Project to be inserted in 44B5-6 by in situ hybridization to polytene chromosomes and the insertion was designated P{lacW}Adk2-44Bk16120, but their complementation tests with Df(2R)CA53, Df(2R)H3C1 and Df(2R)nap16 indicated that the recessive lethality of the insertion chromosome does not map to the 44B region. 
While we cannot explain why probes hybridized to 44B in the BDGP experiment, the sequence and complementation results indicate that P{lacW}k16120 maps to 60B and not 44B. Since localization of this insertion to 44B was the only evidence for an Adk2 gene in 44B, the Adk2-44B gene (FBgn0021824) should be eliminated and the information on P{lacW}k16120 should be merged into the entry for Adk2.