FlyBase Insertion Report: Dmel\P{lacW}Ak2[k16120]

General Information

Symbol

Dmel\P{lacW}Ak2^k16120

Species

D. melanogaster

Name

FlyBase ID

FBti0006368

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

LINE ID

l(2)k16120

Affected gene(s)

Ak2, Ecol\lacZ

Viability / fertility

Associated allele(s)

Ak2^k16120, Ecol\lacZ^Ak2-k16120

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

2R ( 60B4 )

Sequence Location

2R:24,024,374..24,024,374 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:24,024,374..24,024,374 [-]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

60B4 (inferred by FlyBase from sequence location)

Cytological location (reported)

60B_r6 (reported as inferred from sequence location)

(Golden and Cook, 2015.8.6)

44B5-44B6 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

'P{lacW}k16120' was reported by the Berkeley Drosophila Genome Project to be inserted in 44B5-6 by in situ hybridization to polytene chromosomes, but the flanking sequence and complementation results indicate that 'P{lacW}k16120' maps to 60B and not 44B.

(Golden and Cook, 2015.8.6)

Sequence Data

Flanking sequence

AQ026101

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC