UAS-ZucDN: Zuc cDNA with C505 change of A to N was cloned into pattB-UAS vector and 3XHA tag was fused to the C-terminus. The transgene was inserted into attP40 site. UAS-Zuc: Zuc cDNA was cloned into pattB-UAS vector with 3XHA tagged at the C-terminus .The transgene was inserted into attP40 site.