The following information accompanied stocks donated to the Bloomington Stock Center by Alex Kolodkin, Johns Hopkins School of Medicine.
PBac{Sema-2a.TM} is a construct similar to PBac{Sema-2b.TM} (http://flybase.org/reports/FBtp0070958.html). It expresses Sema-2a protein fused to the transmembrane region of T:Mmus \Cd8a (http://flybase.org/reports/FBgn0063453.html) and T:Avic \GFP (http://flybase.org/reports/FBgn0016110.html) under the control of Sema-2a regulatory sequences. Both constructs were generated for the work described in Wu et al. (http://flybase.org/reports/FBrf0213571.html).
PBac{Sema-2a.TM}VK00023 is a phiC31 integrase-mediated insertion into PBac{y+-attP-9A}VK00023 (http://flybase.org/reports/FBti0076444.html) at 70A2, 3L:13213019 (Release 6).