The following information accompanied stocks donated to the Bloomington Stock Center by John Kiger, University of California at Davis.
The batone mutation (bae1) prevents Bursicon production in certain neurons of the subesophageal ganglion, leading to failure of Bursicon release from abdominal nerves, and preventing wing expansion and other maturation events. The mutation acts by mis-expressing the disco-r gene in those neurons. The responsible mutation does not reside in the coding regions of disco-r, but it has not yet been identified molecularly. RNA-guided Cas9 mutagenesis was used to produce frameshift mutations in the disco-r gene that revert the batone phenotype: the disco-rM22.1 allele combines the bae1 regulatory mutation with a frameshift mutation.
Frameshift mutations were also generated in normal chromosomes by RNA-guided Cas9 mutagenesis. disco-rBM6c and disco-rBM3a are alleles with frameshift mutations in the N-terminal exon.  disco-rAM16.1 is an allele with a frameshift mutation in the C-terminal exon.
Partial sequences showing the mutations in disco-rM22.1, disco-rBM6c and disco-rBM3a are given in the associated file.