The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Larry Marsh, University of California, Irvine.
httKO is a CRISPR-generated replacement of the 1st three exons of htt with 3xP3-DsRED. The allele is viable.
M{UAS-HTT.FL.Q25} expresses full length human HTT with 25 Qs under the control of UAS.
M{UAS-HTT.FL.Q25.K444Q} expresses full length human HTT with 25 Qs and K444 changed to Q under the control of UAS.
M{UAS-HTT.FL.Q25.K444R} expresses full length human HTT with 25 Qs and K444 changed to R under the control of UAS.
M{UAS-HTT.FL.Q25.S13A.S16A} expresses full length human HTT with 25 Qs and both S13 and S16 changed to A under the control of UAS.
M{UAS-HTT.FL.Q25.S13D.S16D} expresses full length human HTT with 25 Qs and both S13 and S16 changed to D under the control of UAS.
M{UAS-HTT.FL.Q120.His} expresses full length human HTT with 120 Qs, tagged at the C-terminus with a removable His tag, under the control of UAS.
M{UAS-HTT.FL.Q120.K444Q} expresses full length human HTT with 120 Qs and K444 changed to Q under the control of UAS.
M{UAS-HTT.FL.Q120.K444R} expresses full length human HTT with 120 Qs and K444 changed to R under the control of UAS.
M{UAS-HTT.FL.Q120.S13A.S16A} expresses full length human HTT with 120 Qs and both S13 and S16 changed to A under the control of UAS.
M{UAS-HTT.FL.Q120.S13D.S16D} expresses full length human HTT with 120 Qs and both S13 and S16 changed to D under the control of UAS.
P{UAS-HTT.FL.Q120.His} expresses full length human HTT with 120 Qs, tagged at the C-terminus with a removable His tag, under the control of UAS.
M{Cg-HTT.ex1.Q120} expresses human HTT exon 1 with 120 Qs in the fat body under the control of 2.7kb regulatory region located between the Cg25C and vkg.
M{UAS-HTT.ex1.Q25} expresses human HTT exon 1 with 25 Qs under the control of UAS.
M{UAS-HTT.ex1.Q25.S13A.S16A} expresses human HTT exon 1 with 25 Qs and both S13 and S16 changed to A under the control of UAS.
M{UAS-HTT.ex1.Q25.S13D.S16D} expresses human HTT exon 1 with 25 Qs and both S13 and S16 changed to D under the control of UAS.
M{UAS-HTT.ex1.Q97.T3A} expresses human HTT exon 1 with 97 Qs with T3 changed to A under the control of UAS.
M{UAS-HTT.ex1.Q97.T3D} expresses human HTT exon 1 with 97 Qs with T3 changed to D under the control of UAS.
P{UAS-HTT.ex1.Q50} expresses human HTT exon 1 with 50 Qs under the control of UAS.
P{UAS-HTT.ex1.Q97.3KQ} expresses human HTT exon 1 with 97 Qs with K6, K9 and K15 all changed to Q under the control of UAS.
P{UAS-HTT.ex1.Q97.3KR.S16D} expresses human HTT exon 1 with 97 Qs with K6, K9 and K15 changed to Q and S16 changed to D under the control of UAS.
P{UAS-HTT.ex1.Q97.K15R} expresses human HTT exon 1 with 97 Qs with K15 changed to R under the control of UAS.
P{UAS-HTT.ex1.Q97.K6R.K9R} expresses human HTT exon 1 with 97 Qs with both K6 and K9 changed to R under the control of UAS.
P{UAS-HTT.ex1.Q97.K6R} expresses human HTT exon 1 with 97 Qs with K6 changed to R under the control of UAS.
P{UAS-HTT.ex1.Q97.K9R} expresses human HTT exon 1 with 97 Qs with K9 changed to R under the control of UAS.
M{UAS-HTT.ex1.Q120.LUM} expresses human HTT exon 1 with 120 Qs, tagged at the C-terminus with a Lumio tetracysteine tag, under the control of UAS.
M{UAS-HTT.ex1.Q120.S13A.S16A} expresses human HTT exon 1 with 120 Qs and both S13 and S16 changed to A under the control of UAS.
M{UAS-HTT.ex1.Q120.S13D.S16D} expresses human HTT exon 1 with 120 Qs and both S13 and S16 changed to D under the control of UAS.
M{UAS-HTT.108.Q120.HA} expresses the N-terminal 108 amino acids of human HTT with 120 Qs, tagged at the C-terminus with HA, under the control of UAS.
M{UAS-HTT.118.Q120.HA} expresses the N-terminal 118 amino acids of human HTT with 120 Qs, tagged at the C-terminus with HA, under the control of UAS. Fragment mimics mouse HTT 'shortstop' model.
M{UAS-HTT.118.Q120.LUM} expresses the N-terminal 118 amino acids of human HTT with 120 Qs, tagged at the C-terminus with a Lumio tetracysteine tag, under the control of UAS. Fragment mimics mouse HTT 'shortstop' model.
M{UAS-HTT.118.Q120} expresses the N-terminal 118 amino acids of human HTT with 120 Qs under the control of UAS. Fragment mimics mouse HTT 'shortstop' model.
M{UAS-HTT.171.Q120} expresses the N-terminal 171 amino acids of human HTT with 120 Qs under the control of UAS. Fragment mimics mouse HTT construct described in Schilling et al., 2007, J. Neuropath & Exp Neuro, 66(4):313.
M{UAS-HTT.171.Q120.HA} expresses the N-terminal 171 amino acids of human HTT with 120 Qs, tagged at the C-terminus with HA, under the control of UAS. Fragment mimics mouse HTT construct described in Schilling et al., 2007, J. Neuropath & Exp Neuro, 66(4):313.
M{UAS-HTT.171.Q120.LUM} expresses the N-terminal 171 amino acids of human HTT with 120 Qs, tagged at the C-terminus with a Lumio tetracysteine tag, under the control of UAS. Mimics mouse HTT construct described in Schilling et al., 2007, J. Neuropath & Exp Neuro, 66(4):313.
M{UAS-HTT.469.Q120.HA} expresses the N-terminal 469 amino acids of human HTT with 120 Qs, tagged at the C-terminus with HA, under the control of UAS. Fragment mimics a naturally occurring fragment ending at a calpain site.
M{UAS-HTT.469.Q120.LUM} expresses the N-terminal 469 amino acids of human HTT with 120 Qs, tagged at the C-terminus with a Lumio tetracysteine tag, under the control of UAS. Fragment mimics a naturally occurring fragment ending at a calpain site.
M{UAS-HTT.586.Q120.HA} expresses the N-terminal 586 amino acids of human HTT with 120 Qs, tagged at the C-terminus with HA, under the control of UAS. Fragment mimics a naturally occurring fragment ending at a caspase-6 site.
M{UAS-HTT.586.Q120.LUM} expresses the N-terminal 586 amino acids of human HTT with 120 Qs, tagged at the C-terminus with a Lumio tetracysteine tag, under the control of UAS. Fragment mimics a naturally occurring fragment ending at a caspase-6 site.
M{UAS-HTT.1220.Q120} expresses the N-terminal 1220 amino acids of human HTT with 120 Qs under the control of UAS.
M{UAS-HTT.1220.Q25} expresses the N-terminal 1220 amino acids of human HTT with 25 Qs under the control of UAS.
P{UAS-ATXN2.117Q} expresses full length human Ataxin-2 (ATXN2) with 117Qs under the control of UAS.
The following insertions on the second chromosome are viable:
M{UAS-HTT.513.Q120}ZH-51D
The following insertions on the second chromosome are viable and fertile:
P{UAS-HTT.ex1.Q97.3KR}2
P{UAS-ATXN2.117Q}8B
P{UAS-Q108}16
P{UAS-HTT.FL.Q120.His}2
M{UAS-HTT.ex1.Q120}ZH-51D
M{UAS-HTT.586.Q120}ZH-51D
M{UAS-HTT.552.Q120}ZH-51D
M{UAS-HTT.536.Q120}ZH-51D
M{UAS-HTT.469.Q25}ZH-51D
M{UAS-HTT.469.Q120}ZH-51D
M{UAS-HTT.108.Q120}ZH-51D
M{UAS-HTT.108.Q120.HA}ZH-51D
M{UAS-HTT.118.Q120.HA}ZH-51D
M{UAS-HTT.118.Q120.LUM}ZH-51D
M{UAS-HTT.118.Q120}ZH-51D
M{UAS-HTT.1220.Q120}ZH-51D
M{UAS-HTT.1220.Q25}ZH-51D
M{UAS-HTT.171.Q120.HA}ZH-51D
M{UAS-HTT.171.Q120}ZH-51D
M{UAS-HTT.469.Q120.HA}ZH-51D
M{UAS-HTT.469.Q120.LUM}ZH-51D
M{UAS-HTT.586.Q120.HA}ZH-51D
M{UAS-HTT.586.Q120.LUM}ZH-51D
M{UAS-HTT.ex1.Q120.LUM}ZH-51D
M{UAS-HTT.ex1.Q120.S13A.S16A}ZH-51D
M{UAS-HTT.ex1.Q120.S13D.S16D}ZH-51D
M{UAS-HTT.ex1.Q25.S13A.S16A}ZH-51D
M{UAS-HTT.ex1.Q25.S13D.S16D}ZH-51D
M{UAS-HTT.ex1.Q25}ZH-51D
M{UAS-HTT.FL.Q120.His}ZH-51D
M{UAS-HTT.FL.Q120.K444Q}ZH-51D
M{UAS-HTT.FL.Q120.K444R}ZH-51D
M{UAS-HTT.FL.Q120.S13A.S16A}ZH-51D
M{UAS-HTT.FL.Q120.S13D.S16D}ZH-51D
M{UAS-HTT.FL.Q25.K444Q}ZH-51D
M{UAS-HTT.FL.Q25.K444R}ZH-51D
M{UAS-HTT.FL.Q25.S13A.S16A}ZH-51D
M{UAS-HTT.FL.Q25.S13D.S16D}ZH-51D
M{UAS-HTT.FL.Q25}ZH-51D
M{UAS-HTT.171.Q120.LUM}ZH-51D
The following insertions on the third chromosome are viable:
P{UAS-HTT.ex1.Q97.K6R.K9R}1
The following insertions on the third chromosome are viable and fertile:
P{UAS-HTT.ex1.Q93}4F1
P{UAS-HTT.ex1.Q97}3
P{UAS-HTT.ex1.Q20}111F1L
P{UAS-HTT.ex1.Q50}Y4
P{UAS-HTT.ex1.Q97.3KQ}3
P{UAS-HTT.ex1.Q97.3KR.S16D}1
P{UAS-HTT.ex1.Q97.K15R}1
P{UAS-HTT.ex1.Q97.K6R}7
M{Cg-HTT.ex1.Q120}ZH-86Fb
M{UAS-HTT.ex1.Q97.T3A}ZH-86Fb
M{UAS-HTT.ex1.Q97.T3D}ZH-86Fb
