The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Peter Soba, University of Hamburg.
P{20XUAS-Phobos.CA.mCer3} and PBac{20XUAS-Phobos.CA.mCer3} are both composed of a blue-shifted anion channelrhodopsin (iC++ with additional mutations T159G and G163A) with a C128A mutation that allows switching from an open to closed state with short light pulses. It is expressed under the control of UAS and is tagged at the C-terminal end with mCerulean3.
P{20XUAS-Phobos.CA.mCer3}attP2 is a homozygous viable and fertile insertion on the third chromosome.
PBac{20XUAS-Phobos.CA.mCer3}VK00037 is an insertion on the second chromosome.
P{20XUAS-Aurora.mCer3} is composed of a red-shifted anion-selective channelrhodopsin (derived from ReaChR) under the control of UAS. It is tagged at the C-terminal end with mCerulean3.
P{20XUAS-Aurora.mCer3}attP2 is a homozygous viable insertion on the third chromosome.
P{20XUAS-iChloC.tdTom} is composed of the anion-selective channelrhodopsin iChloC under the control of UAS. It is tagged at the C-terminal end with tdTomato.
P{20XUAS-iChloC.tdTom}attP2 is a homozygous viable and fertile insertion on the third chromosome.