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General Information
Symbol
Dmel\PBac{y+-attP-3B}VK00037
Species
D. melanogaster
Name
FlyBase ID
FBti0076455
Description
Inserted Element
Uses
Affected gene(s)
Viability / fertility
viable
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
2L ( 22A3 )
Sequence Location
2L:1,582,820..1,582,820 [+]
Target / Docking site
Member of Large Scale Dataset(s)
Description

A set of over 400 interchromosomal duplications tiling the majority of the second and third chromosomes from Drosophila melanogaster. The transgenic lines were created via site-specific transgenesis mediated by phiC31 integrase and confirmed by PCR genotyping using clone-specific primers. All second chromosome BACs are inserted at the VK31 docking site on the third chromosome, while all third chromosome BACs are inserted at VK37 on the second chromosome. This set of BACs overlap by an average of 5-10 kb and are primarily >80 kb in size (BAC ID starts with CH321), although some 20 kb BACs (BAC ID starts with CH322) are also present in this collection.

Docking-site insertion on 2L used for one line.

A set of ~400 overlapping Pacman BAC clones was used to create small duplications (average length 88 kb) covering most of the 22-Mb sequenced portion of the X chromosome. Using the Pacman system (FBrf0194996), the BAC clones were inserted into an attP docking site on chromosome arm 3L; available as homozygous or balanced heterozygous stocks.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:1,582,820..1,582,820 [+]
Orientation
Cytological location (computed by FlyBase)
22A3 (inferred by FlyBase from sequence location)
Cytological location (reported)
22A3 (reported as inferred from sequence location)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Location-dependent role
Size (Kb)
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene
Alleles and Phenotypes
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (4)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Symbol Synonym
PBAC{y+-attP}VK00037
PBac{y+-attP-3B}CG14351VK00037
PBac{y+-attP-3B}hafVK00037
PBac{yellow+-attP-3B}VK00037
Name Synonyms
Secondary FlyBase IDs
    References (141)