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Reference
Citation
Venken, K.J., He, Y., Hoskins, R.A., Bellen, H.J. (2006). P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster.  Science 314(5806): 1747--1751.
FlyBase ID
FBrf0194996
Publication Type
Research paper
Abstract

We describe a transgenesis platform for Drosophila melanogaster that integrates three recently developed technologies: a conditionally amplifiable bacterial artificial chromosome (BAC), recombineering, and bacteriophage PhiC31-mediated transgenesis. The BAC is maintained at low copy number, facilitating plasmid maintenance and recombineering, but is induced to high copy number for plasmid isolation. Recombineering allows gap repair and mutagenesis in bacteria. Gap repair efficiently retrieves DNA fragments up to 133 kilobases long from P1 or BAC clones. PhiC31-mediated transgenesis integrates these large DNA fragments at specific sites in the genome, allowing the rescue of lethal mutations in the corresponding genes. This transgenesis platform should greatly facilitate structure/function analyses of most Drosophila genes.

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PubMed Central ID
Related Publication(s)
Personal communication to FlyBase

P"["acman"]" transformation system, additional information.
Venken, 2007.4.11, P"["acman"]" transformation system, additional information. [FBrf0195418]

Note

Tinkering with the fly genome.
Potash, 2007, Nat. Methods 4(2): 114 [FBrf0193513]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Science
    Title
    Science
    Publication Year
    1895-
    ISBN/ISSN
    0036-8075 1095-9203
    Data From Reference
    Alleles (34)
    Genes (18)
    Molecular Constructs (4)
    Natural transposons (2)
    Insertions (33)
    Experimental Tools (1)
    Transgenic Constructs (27)