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FB2021_04, released August 17, 2021

Insertion: Dmel\PBac{y+-attP-3B}VK00033

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General Information
Symbol
Dmel\PBac{y+-attP-3B}VK00033
Species
D. melanogaster
Name
FlyBase ID
FBti0076453
Feature type
transposable_element_insertion_site
Also Known As
VK00033
Description
Inserted Element
PBac{y+-attP-3B}
Uses
Affected gene(s)
CG42747
Viability / fertility
viable
Associated allele(s)
Stock availability
6 publicly available
LINE ID
Genomic Location
Chromosomal Location
3L ( 65B2 )
Sequence Location
3L:6,442,676..6,442,676 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
Dps(X)DC_set1Dps_BAC_Retrofit
Description

A set of over 60 interchromosomal duplications derived from site-specific integration of retrofitted BACs, generated for the Bloomington Stock Center collection. BAC clones were 'retrofitted' using the plasmid TKVBL-w+ so that they were competent for Pacman style transgenesis, and inserted by phiC31-mediated recombination into the VK33 docking site on the third chromosome.

(Venken et al., 2012.4.25)

Docking-site insertion on 2L used for one line.

(Popodi et al., 2010-)

A set of ~400 overlapping Pacman BAC clones was used to create small duplications (average length 88 kb) covering most of the 22-Mb sequenced portion of the X chromosome. Using the Pacman system (FBrf0194996), the BAC clones were inserted into an attP docking site on chromosome arm 3L; available as homozygous or balanced heterozygous stocks.

(Venken et al., 2010)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:6,442,676..6,442,676 [+]
(Venken, 2006)
Orientation

+

(Venken, 2006)
Cytological location (computed by FlyBase)
65B2 (inferred by FlyBase from sequence location)
Cytological location (reported)
65B2 (reported as inferred from sequence location)
(Venken, 2006)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
PBac{y+-attP-3B}
(Venken, 2007.4.11, Venken, 2006, Venken et al., 2006)
Location-dependent role
Size (Kb)
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene
CG42747
(Venken et al., 2010)
Alleles and Phenotypes
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (6)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Insertion in a 5' intron of CG42747.

(Venken et al., 2010)
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
33
(Venken et al., 2006)
PBac{y+-attP-3B} (chromosome 3 L, 65B2)
(Dewald et al., 2014)
PBac{y+-attP-3B}VK00033
(Eisman et al., 2015, Bellen, 2014.10.8, FlyBase, 1992-)
PBac{yellow+-attP-3B}VK00033
(Drosophila Stock Center, 2008.2.28)
VK00033
(Thor, 2016.1.14, Eisman et al., 2015, Alfieri et al., 2013, Venken, 2007.4.11, Venken, 2006)
Name Synonyms
Secondary FlyBase IDs
    References (170)