A set of over 60 interchromosomal duplications derived from site-specific integration of retrofitted BACs, generated for the Bloomington Stock Center collection. BAC clones were 'retrofitted' using the plasmid TKVBL-w+ so that they were competent for Pacman style transgenesis, and inserted by phiC31-mediated recombination into the VK33 docking site on the third chromosome.
Docking-site insertion on 2L used for one line.
A set of ~400 overlapping Pacman BAC clones was used to create small duplications (average length 88 kb) covering most of the 22-Mb sequenced portion of the X chromosome. Using the Pacman system (FBrf0194996), the BAC clones were inserted into an attP docking site on chromosome arm 3L; available as homozygous or balanced heterozygous stocks.
+
Insertion in a 5' intron of CG42747.