We made the Lrp4dalek deletion using non-homologous end joining with two CRISPR sites. The primers recognize the following sites: Start PAM site primer: 15912047 to 15912066 End PAM site primer: 15919938 to 15919919 So the estimated deletion should be from about X:15912066 to 15919919 (2 bp upstream of the start codon and 34 bp downstream of the stop codon).