FlyBase Allele Report: Dmel\Lrp4[dalek]

General Information

Symbol

Dmel\Lrp4^dalek

Species

D. melanogaster

Name

FlyBase ID

FBal0326703

Feature type

allele

Associated gene

Dmel\Lrp4

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Mosca et al., 2017)

Mutagen

CRISPR/Cas9

(Mosca et al., 2017)

Progenitor genotype

Mi{ET1}Lrp4^MB03015

(Mosca et al., 2017)

Cytology

Description

Deletion of the entire coding region (as well as the Mi{ET1}Lrp4^MB03015 insertion between exons 5 and 6 present in the progenitor).

(Mosca et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:15,912,066..15,919,919 [+]

Comment:

The CRISPR-mediated deletion of the entire coding region of Lrp4 is estimated to extend from 2 bp upstream of the Lrp4 start codon to 34 bp downstream of the stop codon.

(Mosca, 2018.1.22)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage

(Mosca et al., 2017)

decreased body size | adult stage

(Mosca et al., 2017)

viable | recessive

(Mosca et al., 2017)

Phenotype Manifest In

adult antennal lobe projection neuron

(Mosca et al., 2017)

adult olfactory receptor neuron

(Mosca et al., 2017)

presynaptic active zone | adult stage

(Mosca et al., 2017)

synapse | adult stage

(Mosca et al., 2017)

T-bar | adult stage

(Mosca et al., 2017)

Detailed Description

Statement

Reference

Lrp4^dalek mutants are viable with a slightly reduced body size and show reduction of synapse number (assessed by counting active zone marker puncta) in VA1v olfactory receptor neuron (ORN) axon terminals in females and in ORN axon terminals projecting to the VA1d, DA1, DL4 and DM6 glomeruli as well as in the excitatory neurons projecting to the lateral horn, while no such decrease is observed in the Gad1-positive inhibitory neurons projecting to the DA1 glomerulus or in the inhibitory neurons projecting to the lateral horn. The neurite volume is not affected in any of the neurons except for a slight decrease in the inhibitory lateral horn projecting neurons. On the ultrastructural level, the mutant ORN terminals have slightly increased perimeter and show significant decrease in the T-bar number the majority of which display various morphological defects (including lacking tops, detached, misshapen and frequent multiple T-bars).

No synaptic morphological defects are observed in the VA1v ORNs in Lrp4^dalek/+ heterozygous adults.

(Mosca et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lrp4^dalek has abnormal neuroanatomy | adult stage phenotype, suppressible by Srpk79D^P10036/Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Other

Statement

Reference

Lrp4^dalek, Srpk79D^P10036/Srpk79D[+] has abnormal neuroanatomy phenotype

(Mosca et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lrp4^dalek has adult olfactory receptor neuron phenotype, suppressible by Srpk79D^P10036/Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Lrp4^dalek has synapse | adult stage phenotype, suppressible by Srpk79D^P10036/Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Lrp4^dalek has presynaptic active zone | adult stage phenotype, suppressible by Srpk79D^P10036/Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

NOT Suppressor of

Statement

Reference

Lrp4^dalek is a non-suppressor of photoreceptor cell R7 | adult stage phenotype of Scer\GAL4^GMR.PF, loaf^JF03040

(Douthit et al., 2021)

Lrp4^dalek is a non-suppressor of axon terminus | adult stage phenotype of Scer\GAL4^GMR.PF, loaf^JF03040

(Douthit et al., 2021)

Lrp4^dalek is a non-suppressor of outer medulla | adult stage phenotype of Scer\GAL4^GMR.PF, loaf^JF03040

(Douthit et al., 2021)

Other

Statement

Reference

Lrp4^dalek, Srpk79D^P10036/Srpk79D[+] has synapse | adult stage phenotype

(Mosca et al., 2017)

Lrp4^dalek, Srpk79D^P10036/Srpk79D[+] has adult olfactory receptor neuron phenotype

(Mosca et al., 2017)

Lrp4^dalek, Srpk79D^P10036/Srpk79D[+] has presynaptic active zone | adult stage phenotype

(Mosca et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Neither Lrp4^dalek/+ nor Srpk79D^P10036/+ single heterozygous adults display any defects in the synapse morphology in the VA1v olfactory receptor neurons but in Lrp4^dalek/+;Srpk79D^P10036/+ double heterozygotes the synapse number (assessed by counting active zone marker puncta) is significantly decreased compared to controls.

The reduced active zone number in the VA1v olfactory receptor neurons observed in Lrp4^dalek homozygous mutants is restored by expression of Srpk79D^P10036 under the control of Scer\GAL4^peb-GAL4 in the mutant background.

(Mosca et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lrp4^dalek

(Douthit et al., 2021, Mosca, 2018.1.22)

lrp4^dalek

(DePew et al., 2024, Mosca et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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