FB2026_02 , released June 18, 2026
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Grabbe, C. (2018.7.10). Urm1 and Uba4 constructs from Caroline Grabbe. 
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FBrf0239409
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Personal communication to FlyBase
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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Caroline Grabbe, Umea University.
P{UAS-Urm1.WT}2 is a second chromosome insertion, homozygous viable and fertile.
P{UAS-Urm1.WT}3 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-Flag-Urm1.WT}2 is a second chromosome insertion, homozygous viable and fertile.
P{UAS-Flag-Urm1.WT}3 is a third chromosome insertion.
P{UAS-Urm1.DeltaGG}2 is a second chromosome insertion, homozygous viable and fertile.
P{UAS-Urm1.DeltaGG}3 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-Flag-Urm1.DeltaGG}3 is a third chromosome insertion.
P{UAS-Flag-Urm1.DeltaGG}2 is a second chromosome insertion, homozygous viable.
P{UAS-Uba4.WT}3 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-Uba4.WT}2 is a second chromosome insertion, homozygous viable and fertile.
P{UAS-Uba4.WT}1 is a first chromosome insertion, homozygous viable and fertile.
P{UAS-Myc-Uba4.WT}3 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-Myc-Uba4.WT}2 is a second chromosome insertion, homozygous viable and fertile.
Uba4n29 is a potential null allele generated by imprecise excision of P{GSV6}GS11970, resulting a 963 bp deletion in the coding region of Uba4, including the initiation codon and the first 242 amino acids. 
The following constructs were generated similarly to that of P{UAS-Urm1.WT} as reported in FBrf0231575 :
P{UAS-HA-Urm1.WT}is a wildtype Urm1 cloned in pTHW.
P{UAS-HA-Urm1.DeltaGG} is a mutant Urm1.DeltaGG cloned in pTHW.
P{UAS-EGFP-Urm1.WT} is a wildtype Urm1 cloned in pTGW.
P{UAS-EGFP-Uba4.WT} is a wildtype Uba4 cloned in pTGW.
The following insertions are unpublished:
P{UAS-HA-Urm1.WT}3 is a third chromosome insertion.
P{UAS-HA-Urm1.DeltaGG}2 is a second chromosome insertion, homozygous viable.
P{UAS-EGFP-Urm1.WT}2 is a second chromosome insertion, homozygous viable and fertile.
P{UAS-EGFP-Uba4.WT}2 is a second chromosome insertion, homozygous viable.
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    English
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