Zhao, Y., Bushey, D., Zhao, Y., Schreiter, E.R., Harrison, D.J., Wong, A.M., Campbell, R.E. (2018). Inverse-response Ca2+ indicators for optogenetic visualization of neuronal inhibition. Sci. Rep. 8(1): 11758.
FlyBase ID
FBrf0239772
Publication Type
Research paper
Abstract
We have developed a series of yellow genetically encoded Ca2+ indicators for optical imaging (Y-GECOs) with inverted responses to Ca2+ and apparent dissociation constants (Kd') ranging from 25 to 2400 nM. To demonstrate the utility of this affinity series of Ca2+ indicators, we expressed the four highest affinity variants (Kd's = 25, 63, 121, and 190 nM) in the Drosophila medulla intrinsic neuron Mi1. Hyperpolarization of Mi1 by optogenetic stimulation of the laminar monopolar neuron L1 produced a decrease in intracellular Ca2+ in layers 8-10, and a corresponding increase in Y-GECO fluorescence. These experiments revealed that lower Kd' was associated with greater increases in fluorescence, but longer delays to reach the maximum signal change due to slower off-rate kinetics.
