The following information accompanied stocks donated to the Bloomington Stock Center by Julian Ng, University of Cambridge.
P{UAS-wit.Venus} expresses wit protein with a C-terminal Venus tag under UASt control. The transgene was constructed by placing full-length wit-PA sequence into pP{TMV}.
P{UAS-wit.Venus}1M2 is a second chromosome insertion.
P{UAS-wit.Venus}6F is a third chromosome insertion.
P{UAS-put.myc} expresses put protein with six tandem copies of myc at the C-terminus under UASt control. The transgene was constructed by placing full-length put-PA sequence into pP{TWM}.
P{UAS-put.myc}4M is a second chromosome insertion.
P{UAS-put.KR.myc} expresses put protein with a mutated ATP-binding site with with six tandem copies of myc at the C-terminus under UASt control. The transgene was constructed by placing full-length, mutated put-PA sequence into pP{TWM}.
P{UAS-put.KR.myc}5M is a second chromosome insertion.
P{UAS-bsk.D160N.E328A.E330A.Venus} expresses bsk protein with D160N, E328A and E330A substitutions and a C-terminal Venus tag under UASt control. The substitutions disrupt interactions with Mkk4 and Aplip1. The transgene was constructed by placing full-length, mutated bsk-PB sequence into pP{TMV}.
P{UAS-bsk.D160N.E328A.E330A.Venus}1 is a homozygous and hemizygous viable and fertile insertion at 10F1 on the X chromosome.
P{UAS-RhoGEF2.GFP} expresses RhoGEF2 protein (PE isoform) with a C-terminal GFP tag under UASt control. The protein sequence is:
MDDPSIKKRLLDLYTDEHEYDEVQEIPEESSIQPPETSTSHTSTNGSSHSGPGTATGPGATSAGPSAGAPQSPVIVVDSV
PELPAPKQKSVKNSKSKQKQKQLANKSKIPRSPSLASSLSSLASSLSGHRDRDKDRDKDRENQNAVPPQTPPLPPSYKQN
QMNGDSTAAAGGGVSAPATPTTANNNNASHNNGSIMGGGVQLNQSDNSNPVLQAPGERSSLNLLTPLSRDLSGGHTQEST
TPATTPSTPSLALPKNFQYLTLTVRKDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAGDMILRVNGHEVRLEKHPTVV
GLIKASTTVELAVKRSQKLTRPSSVSVVTPSTPILSGRDRTASITGPQPVDSIKRREMETYKIQTLQKMLEQEKLNLERL
KSDQNNPSYKLSEANIRKLREQLHQVGAEALGQTPNLGKNKHRRVGSSPDNMHPRHPDRLTKTTSGSWEIVEKDGESSPP
GTPPPPYLSSSHMTVLEDPNENSRGAAAAGPGVFIESHQFTPMAGASSPIPISLHSSHMHAAQSNDTQKEIISMEDENSD
LDEPFIDENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFYLITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSW
YRQDESLAREVDNVLQLEYDKVEILRTVFLRSRKRAKDLISEQLREFQQKRTAGLGTIYGPTDDKLAEAKTDKLREQIID
KYLMPNLHALIEDENGSPPEDVRKVALCSALSTVIYRIFNTRPPPSSIVERVHHFVSRDKSFKSRIMGKNRKMNVRGHPL
VLRQYYEVTHCNHCQTIIWGVSPQGYHCTDCKLNIHRQCSKVVDESCPGPLPQAKRLAHNDKISKFMGKIRPRTSDVIGN
EKRSRQDEELDVELTPDRGQASIVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMD
LSTSVASTTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQHPKHKSAPVSVNRSESYKERLSNKRNRNSRRKTSDPS
LSSRPNDEQLDLGLSNATYVGSSNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHAQQYCQQD
SFQAGLAGAAGSSAASNSSFWNAGHPLPVARWTLESEDEDDVNEADWSSMVAAEVLAALTDAEKKRQEIINEIYQTERNH
VRTLKLLDRLFFLPLYESGLLSQDHLLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLCE
FAAQFCARQQIALEALKEKRNKDEMLQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFENLYKVTVRLLPENTTEA
EAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSYDKEEFKKLDLTQHHLIHDGNLTIKKNPSVQLHGLLFEN
MIVLLTKQDDKYYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKMKTSQMLELRAPSSSECKTWFKHFSD
VAARQSKNRSKNASSNHDTSISDPALAAIPHSNTKESLELSTDTVQPLAATATLTTTPLAPMLPIATVTPAPATNNSNVS
SLTGVQLRNPQRDATASESDADYVNTPKPRSSQNEVNRTMSIRSTGEPIQKYSANGTEANDVTLRHSQSTRESVRPGSTG
EERNSTYGMVGGNSKRDSASIVCSNNSNNTRTLLMQSPLVDPTAIQVSISPAHTAEPVLTPGEKLRRLDASIRNDLLEKQ
KIICDIFRLPVEHYDQIVDIAMMPEAPKDSADIALAAYDQIQTLTKMLNEYMHVTPEQEVSAVSTAVCGHCHEKEKLRKK
VAPSSSFSSSPPPLPPPNRQHAQAQAQIPPSRLMPKLQTLDLDEVAIHEDDDGYCEIDELRLPAIPSKPHERPTTPLAPF
NTEPKTSQSVIDASKRQSTDAVPEGLLEQEPLEGDKTETKGEDNEVKTVPSDKLSESCNEERQCVEADITKEVADPTTSK
NEAAASVDELPSQSREIKTAENASKSVADKKEDNEETIEEGVASTVDSSTQTSPTESPKETDKLTGGSSSTCGPNRIQHA
SVLEPSVPCHALSSIVTILNEQISMLLPKINERDMERERLRKENQHLRELLSALHDRQRVDEVKETPFDLKKLMHAEDVE
FDDDIDAISNSSLTPTPTPIPTASPSASGQVETAEAMRITSTEDEESRMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
EGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAE
VKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDG
PVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSRGPFEGKPIPNPLLGLDSTRTG
P{UAS-RhoGEF2.GFP}9F2 is a second chromosome insertion.